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现货供应外周血单核细胞（PBMC)分离管
1667 人阅读发布时间：2012-02-13 15:32
现货供应德国kisker产外周血单核细胞（PBMC)分离管，规格有50ml与15ml两种， 10个起订。
The 50 ml and 15 ml blood-separation-filter tubes are designed to separate mononuclear cells (MNC). This method has been developed by ESQUIRE(Leuco-sep).
Protocol to be performed under normal room temperature.
1. Pipet 16 ml Lymphoprep* into the BLOOD-SEPARATION tube. (3ml for 15ml tube)
2. Centrifuge at 1000 G for 30 seconds at room temp.
3. Pour 15-30 ml freshly anticoagulated blood into the tube(3-8ml for 15ml tube). The blood does not have to be diluted, but can be diluted directly into the tube with a balanced salt solution.
4. Centrifuge at 1000 G for 10 min. (or 800 G for 15 min.)
5. After centrifugation the sequence of the tube content will be as follows:
Plasma-MNC-Lymphoprep-Filter-Lymphoprep-Erythrocytes.
6. MNC can be collected and transfered by a Pasteur pipet,or directly poured to another tube.
7. Wash the MNC by adding 10 ml PBS and resuspend the cells by gentle aspiration with a pasteur pipet. Centrifuge at 250 G for 10 minutes at room temperature.
8. Repeat step twice, resuspending the pellet in 5 ml PBS-buffer.
9. The Lymphozites are now ready to pickup and stored in cryogenic vials.
*Separation-solution is available as: Lymphoprep (Nycomed Amersham)
The 50 ml and 15 ml blood-separation-filter tubes are designed to separate mononuclear cells (MNC). This method has been developed by ESQUIRE(Leuco-sep).
Protocol to be performed under normal room temperature.
1. Pipet 16 ml Lymphoprep* into the BLOOD-SEPARATION tube. (3ml for 15ml tube)
2. Centrifuge at 1000 G for 30 seconds at room temp.
3. Pour 15-30 ml freshly anticoagulated blood into the tube(3-8ml for 15ml tube). The blood does not have to be diluted, but can be diluted directly into the tube with a balanced salt solution.
4. Centrifuge at 1000 G for 10 min. (or 800 G for 15 min.)
5. After centrifugation the sequence of the tube content will be as follows:
Plasma-MNC-Lymphoprep-Filter-Lymphoprep-Erythrocytes.
6. MNC can be collected and transfered by a Pasteur pipet,or directly poured to another tube.
7. Wash the MNC by adding 10 ml PBS and resuspend the cells by gentle aspiration with a pasteur pipet. Centrifuge at 250 G for 10 minutes at room temperature.
8. Repeat step twice, resuspending the pellet in 5 ml PBS-buffer.
9. The Lymphozites are now ready to pickup and stored in cryogenic vials.
*Separation-solution is available as: Lymphoprep (Nycomed Amersham)